Reliable peristaltic pumps for continuous cultures

  • Highly reliable pumps with reproducible flow rates for bioprocessing techniques
  • Unique tubing compression technique for long tubing life and long term stability of flow rates even with low cost tubing
  • Flow rates remain constant for several weeks without the risk of tubing rupture in long term experiments like continuous cultures, chemostat, perfusion
  • Variable speed pump for better precision e.g.: speed of the peristaltic pump is regulated to attain the preset value of pH, during the addition of acid/base to the medium
  • Stackable and extremely compact peristaltic pump saves valuable bench-space in your laboratory
  • Flow rate programming (up to 99 steps) and automatic switch-on and switch-off without using any timer e.g. programmed feed profile
  • Can also be used as a stand-alone instruments (e.g.: downstream processing experiments)
  • Remote controllable by PC, MINIFOR fermentor-bioreactor, fermentation software SIAM / Fnet


Construction advantages of LAMBDA Peristaltic Pumps

Pump head’s unique compression mechanism reduces the mechanical strain on the tubing and the tubing compression is kept in the range of elasticity of the tubing.

As a result of LAMBDA's special tubing compression, the lifetime of tubing used (even lowest cost silicone tubing) is greatly extended and the flow rates remain constant for several weeks without the risk of tubing rupture in long term experiments like continuous cultures, chemostat, perfusion. LAMBDA peristaltic pumps work well with low cost tubing. The economy obtained by using low cost tubing instead of tubing with stoppers or expensive special tubing material formulations pays the peristaltic pump back after the use of only about 80 m of tubing!

Flow rate programming (up to 99 steps) and automatic switch-on and switch-off without using any timer. Flow rate profile could be used for e.g.: pre-program the feed profile for fed-batch culture directly on the feed pump and run it automatically for 'n' number of times.

Unlike the cheap and unreliable fixed speed pumps, the flow rate of LAMBDA peristaltic pumps can be varied in the ratio 1:1000. For example, during the addition of acid into the medium, the peristaltic pump turns at full speed and reduces the speed as the preset value of pH is being attained. The regulation by "soft landing" is much more precise than by merely switching ON and OFF fixed speed pumps.

LAMBDA disagrees with the concept of offering fixed speed pump in fermentation / cell culture, because the peristaltic pump quality and reliability is a crucial factor, which strongly affects the quality and productivity of the cell culture work.

PRECIFLOW

0.2 µl/min - 600 ml/h

PC-Programmable

5 years warranty

MULTIFLOW

0.2 µl/min - 600 ml/h

Programmable

5 years warranty

HIFLOW

1 µl/min - 3,000 ml/h

Programmable

2 years warranty

MAXIFLOW

3 µl/min - 10,000 ml/h

Programmable

2 years warranty

MEGAFLOW

0.02 ml/min - 60 L/hour

Programmable

2 years warranty

Application of peristaltic pumps

LAMBDA Peristaltic Pumps PRECIFLOW, MULTIFLOW, HIFLOW, MAXIFLOW and MEGAFLOW pumps are highly reliable with stable and reproducible flow rates for long term experiments in the laboratory. Pumps are specially developed for long term continuous upstream processes (batch, fed-batch, turbidostat, chemostat, perfusion, 3-stage fermentation) and downstream processes

  • Feed pump and harvest pump (fed-batch culture, continuous culture, chemostat, turbidostat, perfusion)
  • Controlled addition of nutrients, minerals
  • pH control (addition of acid, base, buffers)
  • Antifoam control (addition of antifoam agent)
  • Sampling at particular time interval
  • Feed flow rate profile (e.g.: pre-program the feed profile for fed-batch culture directly on the feed pump and run it automatically for 'n' number of times)
  • Sample addition/injection
  • Quantify the metabolic activity of the culture
  • Media dispenser
  • Process optimization with C-feed in fed batch
  • Pump media for filling agar plates (with foot switch)

Pump-flow INTEGRATOR replaces OD measurement

LAMBDA offers a possibility to access the cell growth kinetics by measuring and recording the amount of acid or base utilized to maintain the pH of the culture constant.

The LAMBDA PUMP-FLOW INTEGRATOR transforms the impulses driving the motor of the pump into a voltage which can be recorded and displayed using the fermentation software (FNet or SIAM).

Cell growth always produces CO2, which is acidic and to maintain the pH constant, this acid (which partially escapes) has to be compensated by the addition of an acid by a pH controller with an acid pump (formation of metabolic acids and bases other than CO2 are of course also taken into account by this measurement globally). In a balanced cell growth, the production of CO2 and the need of pH correcting acid are proportional to the growth (as the sum of all metabolic processes in the cell). Therefore, the amount of added pH correction solution (acid or base) is a precise indication of the growth extent of the culture. The controlled addition of acid equals (in principle) to an analytical titration method and is therefore, very precise.

The quantification of the INTEGRATOR signal can be obtained by using acids of known concentration and by calibrating the pump flow. Then, the value calibrated correlates to the growth extent measured by another suitable growth rate method. Afterwards, the mere signal of the PUMP-FLOW INTEGRATOR allows precise and very inexpensive real growth kinetics. Optical density (OD) measurement of the culture is not precise because the dead cells, air bubbles, precipitates and any other color or turbidity formation also appear as an increase in cell concentration.

The trace obtained by the INTEGRATOR displays also an indication about growth problems, contamination and extent of the biotransformation. It is an important parameter, when cultures have to be compared in series of fermenters / bioreactor.




It is an example of the trace of an acid pump activity transformed by the INTEGRATOR, during biotransformation culture (red trend with two resets). The red trend is the only one which is clearly exponential, as it should be. By totalizing the consumption of acid, the extent of transformation and the immediate state of the culture can be derived. It is important information for the control and reproducibility of different cultures.